Protocol
AICAR (50 mg Vial)
Complete dosage protocol and reconstitution guide for AICAR (50 mg Vial).
Calculator preset
Run the vial math before tracking.
At ~16.67 mg/mL, 1 unit ~ 167 mcg ; 1 mg ~ 6 units and 3 mg ~ 18 units on a U-100 syringe.
01 · At a glance
Quickstart Highlights
AICAR (5-aminoimidazole-4-carboxamide ribonucleoside) is a research compound that activates AMP-activated protein kinase (AMPK) [1] , earning the nickname “exercise pill” after studies showed sedentary animals given AICAR experienced dramatic endurance improvements [2] . AICAR is prohibited in sports (WADA classifies it as a metabolic modulator) and is not approved for therapeutic use in humans [1] . This educational protocol presents a once-daily subcutaneous approach for research purposes only.
02 · Dosing & reconstitution
Dosing & Reconstitution Guide
A practical calculator-ready setup based on the imported protocol reference. Use these values as a math starting point, then verify every entry against your own vial, diluent, and professional guidance.
Standard / Gradual Approach (3 mL = ~16.7 mg/mL)
1,000–3,000 mcg once daily (conservative gradual approach).
Advanced / Aggressive Approach
Reconstitution Steps
- Draw 3.0 mL bacteriostatic water with a sterile syringe.
- Inject slowly down the vial wall; avoid foaming.
- Gently swirl/roll until dissolved (do not shake).
- Label and refrigerate at 2–8 °C (35.6–46.4 °F) , protected from light.
03 · What you'll need
Supplies Needed
Keep this as a planning checklist. TNHL does not sell or verify supplies on this page, and no supplier quality claims are made here.
- 12 weeks (2–5 mg/day advanced) ≈ 8 vials
- 8 weeks: 56 syringes
- 12 weeks: 84 syringes
- 8 weeks (3 vials): 9 mL → 1 × 10 mL bottle
- 12 weeks (8 vials): 24 mL → 3 × 10 mL bottles
- Per week: 14 swabs (2/day)
- 8 weeks: 112 swabs → recommend 2 × 100-count boxes
- 12 weeks: 168 swabs → recommend 2 × 100-count boxes
Protocol Overview
- Goal: Activate AMPK pathways to mimic exercise-like metabolic effects [1] [6] .
- Schedule: Daily subcutaneous injections for 8–12 weeks (research protocols).
- Dose Range: 1,000–3,000 mcg daily (conservative); up to 5,000 mcg in advanced protocols.
- Reconstitution: 3.0 mL per 50 mg vial (~16.7 mg/mL) for precise measurements.
- Storage: Lyophilized frozen at −20 °C (−4 °F); reconstituted refrigerated 2–8 °C (35.6–46.4 °F) for up to 4 weeks [14] .
Dosing Protocol
- Suggested gradual titration approach for research.
- Start: 1,000 mcg daily; increase by 1,000 mcg every 2 weeks as tolerated.
- Target: 2,000–3,000 mcg daily by Weeks 3–8 (conservative).
- Advanced: Up to 5,000 mcg daily if supported by protocol design.
- Frequency: Once per day (subcutaneous); maintain consistent AMPK activation [3] .
- Timing: Any consistent time; rotate injection sites daily.
Storage Instructions
- Lyophilized: freeze at −20 °C (−4 °F) ; after reconstitution, refrigerate at 2–8 °C (35.6–46.4 °F) for up to 4 weeks; avoid freeze–thaw cycles.
- Storage: Lyophilized frozen at −20 °C (−4 °F); reconstituted refrigerated 2–8 °C (35.6–46.4 °F) for up to 4 weeks [14] .
- Keep mixed and unmixed items clearly labeled so inventory and dose logs stay accurate.
04 · Good to know
Important Notes
- This AICAR page is for educational research organization only.
- Confirm route, dose, contraindications, monitoring, storage, and cycle decisions with qualified professional guidance.
- Use sterile technique, rotate sites when applicable, and log missed doses, reactions, and inventory changes.
05 · How it works
How This Works
AICAR (5-aminoimidazole-4-carboxamide ribonucleoside) is a research compound that activates AMP-activated protein kinase (AMPK) [1] , earning the nickname “exercise pill” after studies showed sedentary animals given AICAR experienced dramatic endurance improvements [2] .
AICAR is prohibited in sports (WADA classifies it as a metabolic modulator) and is not approved for therapeutic use in humans [1] .
This educational protocol presents a once-daily subcutaneous approach for research purposes only.
The library page pairs that educational context with calculator-ready vial math, so users can understand the protocol structure and then track their own entered values in one place.
06 · Daily habits
Lifestyle Factors
- Keep meals, training, sleep, and recovery notes in the journal so trends are easier to review.
- Track body metrics only if they are relevant to the user's goal and approved data sources.
- Use consistent timing and logging habits so adherence data is meaningful.
07 · What to expect
Potential Benefits & Side Effects
- AICAR observations should be recorded conservatively: expected research endpoints, tolerability notes, injection-site reactions when applicable, sleep, appetite, energy, mood, and any adverse or unusual response.
- This section is not a claim of therapeutic benefit.
08 · Technique
Injection Technique
- Wash hands with soap and water.
- Wipe the vial stopper and chosen site with alcohol, then let both dry.
- Confirm the vial concentration, calculator result, and intended draw amount before loading the syringe.
- Use the route and needle technique directed by a qualified professional or product instructions.
- For subcutaneous injections, rotate sites and inject slowly to limit leakage and irritation.
- Do not reuse needles or syringes.
- Dispose of sharps in a puncture-resistant container.
- Return stored items promptly to the correct temperature.
- Log dose, site, date, time, inventory change, and any observation.
09 · Evidence