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Protocol

CJC-1295 DAC (5mg Vial)

Complete dosage protocol and reconstitution guide for CJC 1295 DAC (5mg Vial).

Updated November 26, 2025 Research guide Research information only
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Educational reference only. TNHL calculates and organizes user-entered values; it does not diagnose, prescribe, recommend doses, or replace guidance from a qualified professional.
MechanismCJC-1295 DAC is a long-acting synthetic analog of growth hormone-releasing hormone (GHRH). The DAC (Drug Affinity Complex) modification binds reversibly to albumin, extending the peptide’s half-life to approximately 6–8 days [1] .
Dosing300–1000 mcg per injection, twice weekly (gradual titration).
ReconstitutionAdd 2.0 mL diluent to a 5 mg vial.
StorageLyophilized: freeze at −20 °C (−4 °F) ; after reconstitution, refrigerate at 2–8 °C (35.6–46.4 °F) ; avoid freeze–thaw cycles.

Calculator preset

Run the vial math before tracking.

At 2.5 mg/mL, 1 unit = 25 mcg (0.01 mL); 500 mcg = 20 units and 1000 mcg = 40 units on a U-100 syringe (units = dose ÷ 2.5 mg/mL).

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01 · At a glance

Quickstart Highlights

CJC-1295 DAC is a long-acting synthetic analog of growth hormone-releasing hormone (GHRH). The DAC (Drug Affinity Complex) modification binds reversibly to albumin, extending the peptide’s half-life to approximately 6–8 days [1] . By stimulating pituitary GHRH receptors, CJC-1295 DAC increases GH secretion and circulating IGF-1 in a dose-dependent manner [2] . This educational protocol presents a twice-weekly subcutaneous approach using a practical dilution for clear insulin-syringe measurements.

ReconstituteAdd 2.0 mL bacteriostatic water → 2.5 mg/mL (2500 mcg/mL) concentration. Typical
Reference dose300–1000 mcg per injection, twice weekly (gradual titration).
Easy measuringAt 2.5 mg/mL, 1 unit = 25 mcg (0.01 mL); 500 mcg = 20 units and 1000 mcg = 40 units on a U-100 syringe (units = dose ÷ 2.5 mg/mL).
StorageLyophilized: freeze at −20 °C (−4 °F) ; after reconstitution, refrigerate at 2–8 °C (35.6–46.4 °F) ; avoid freeze–thaw cycles.
Start with sterile preparation, storage, and injection-site basics before using any calculator output. TNHL is a tracking and education tool only.

02 · Dosing & reconstitution

Dosing & Reconstitution Guide

A practical calculator-ready setup based on the imported protocol reference. Use these values as a math starting point, then verify every entry against your own vial, diluent, and professional guidance.

Standard / Gradual Approach (2 mL = 2500 mcg/mL)

Phase / setup Dose & frequency Volume / units
Weeks 1–2300 mcg (0.3 mg)12 units (0.12 mL)
Weeks 3–4500 mcg (0.5 mg)20 units (0.20 mL)
Weeks 5–6750 mcg (0.75 mg)30 units (0.30 mL)
Weeks 7–121000 mcg (1 mg)40 units (0.40 mL)

300–1000 mcg per injection, twice weekly (gradual titration).

Reconstitution Steps

  1. Confirm the vial amount, unit, and diluent amount before entering values in the calculator.
  2. Add diluent slowly down the inner wall of the vial when reconstitution is appropriate for the compound.
  3. Swirl or roll gently until dissolved; avoid shaking unless the product instructions specifically say otherwise.
  4. Label the vial with the date and concentration, then store according to the protocol and product instructions.

03 · What you'll need

Supplies Needed

Keep this as a planning checklist. TNHL does not sell or verify supplies on this page, and no supplier quality claims are made here.

Peptide Vials 8 weeks ≈ 3 vials (~10.2 mg total)
  • 12 weeks ≈ 4 vials (~18.2 mg total)
  • 16 weeks ≈ 6 vials (~26.2 mg total)
Insulin Syringes Per week: 2 syringes (twice weekly)
  • 8 weeks: 16 syringes
  • 12 weeks: 24 syringes
  • 16 weeks: 32 syringes
Bacteriostatic Water Use ~2.0 mL per vial for reconstitution.
  • 8 weeks (3 vials): 6 mL → 1 × 10 mL bottle
  • 12 weeks (4 vials): 8 mL → 1 × 10 mL bottle
  • 16 weeks (6 vials): 12 mL → 2 × 10 mL bottles
Alcohol Swabs One for the vial stopper + one for the injection site each injection.
  • Per week: 4 swabs (2
  • per injection × 2 injections)
  • 8 weeks: 32 swabs → recommend 1 × 100-count box
  • 12 weeks: 48 swabs → recommend 1 × 100-count box

Protocol Overview

  • Goal: Support sustained GH and IGF-1 elevation for research into GH-axis effects [1] .
  • Schedule: Twice-weekly subcutaneous injections for 8–12 weeks (extend to 16 weeks if desired).
  • Dose Range: 300–1000 mcg per injection with gradual titration.
  • Reconstitution: 2.0 mL per 5 mg vial (2.5 mg/mL) for accurate unit measurements.
  • Storage: Lyophilized frozen; reconstituted refrigerated; avoid repeated freeze–thaw.

Dosing Protocol

  • Suggested twice-weekly titration approach.
  • Start: 300 mcg per injection twice weekly; increase by ~250 mcg every 2 weeks as tolerated.
  • Target: 750–1000 mcg per injection by Weeks 5–12.
  • Frequency: Twice per week (subcutaneous), spaced 3–4 days apart.
  • Cycle Length: 8–12 weeks; optional extension to 16 weeks.
  • Timing: Consistent timing (e.g., evenings); rotate injection sites.

Storage Instructions

  • Lyophilized: freeze at −20 °C (−4 °F) ; after reconstitution, refrigerate at 2–8 °C (35.6–46.4 °F) ; avoid freeze–thaw cycles.
  • Storage: Lyophilized frozen; reconstituted refrigerated; avoid repeated freeze–thaw.
  • Keep mixed and unmixed items clearly labeled so inventory and dose logs stay accurate.

04 · Good to know

Important Notes

  • Practical considerations for consistency and safety.
  • Use new sterile insulin syringes; dispose in a sharps container.
  • Rotate injection sites (abdomen, thighs, upper arms) to reduce local irritation.
  • Inject slowly; wait a few seconds before withdrawing the needle.
  • Document each dose, injection site, and timing to maintain consistency.
  • The DAC modification provides sustained release; maintain consistent twice-weekly scheduling.
  • The 5 mg vial provides more doses per vial, reducing reconstitution frequency.

05 · How it works

How This Works

CJC-1295 DAC is a long-acting synthetic analog of growth hormone-releasing hormone (GHRH).

The DAC (Drug Affinity Complex) modification binds reversibly to albumin, extending the peptide’s half-life to approximately 6–8 days [1] .

By stimulating pituitary GHRH receptors, CJC-1295 DAC increases GH secretion and circulating IGF-1 in a dose-dependent manner [2] .

The library page pairs that educational context with calculator-ready vial math, so users can understand the protocol structure and then track their own entered values in one place.

06 · Daily habits

Lifestyle Factors

  • Keep meals, training, sleep, and recovery notes in the journal so trends are easier to review.
  • Track body metrics only if they are relevant to the user's goal and approved data sources.
  • Use consistent timing and logging habits so adherence data is meaningful.

07 · What to expect

Potential Benefits & Side Effects

  • CJC-1295 DAC observations should be recorded conservatively: expected research endpoints, tolerability notes, injection-site reactions when applicable, sleep, appetite, energy, mood, and any adverse or unusual response.
  • This section is not a claim of therapeutic benefit.

08 · Technique

Injection Technique

Pre-injection preparation
  • Wash hands with soap and water.
  • Wipe the vial stopper and chosen site with alcohol, then let both dry.
  • Confirm the vial concentration, calculator result, and intended draw amount before loading the syringe.
Injection procedure
  • Use the route and needle technique directed by a qualified professional or product instructions.
  • For subcutaneous injections, rotate sites and inject slowly to limit leakage and irritation.
  • Do not reuse needles or syringes.
Post-injection care
  • Dispose of sharps in a puncture-resistant container.
  • Return stored items promptly to the correct temperature.
  • Log dose, site, date, time, inventory change, and any observation.

09 · Evidence

References

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