Protocol
Epithalon (10mg Vial)
Complete dosage protocol and reconstitution guide for Epithalon (10mg Vial).
Calculator preset
Run the vial math before tracking.
At 5 mg/mL, 1 unit = 50 mcg ; 5 mg = 100 units (1.0 mL) and 10 mg = 200 units (2.0 mL) on a U-100 syringe.
01 · At a glance
Quickstart Highlights
Epitalon (also known as Epithalon) is a synthetic tetrapeptide (Ala-Glu-Asp-Gly) developed from the naturally occurring pineal peptide epithalamin [1] . Research indicates it may support telomerase activation, circadian rhythm regulation, and anti-aging pathways [2] [3] . Clinical studies employ short intermittent cycles (10–20 days) repeated 1–2 times annually [4] , though extended titration protocols are also explored for research purposes.
02 · Dosing & reconstitution
Dosing & Reconstitution Guide
A practical calculator-ready setup based on the imported protocol reference. Use these values as a math starting point, then verify every entry against your own vial, diluent, and professional guidance.
Standard Cyclic Protocol (2 mL = 5 mg/mL)
5–10 mg once daily (subcutaneous injection).
Reconstitution Steps
- Draw 2.0 mL bacteriostatic water with a sterile syringe.
- Inject slowly down the vial wall to avoid foaming.
- Gently swirl or roll until fully dissolved (do not shake vigorously).
- Label with reconstitution date and refrigerate at 2–8 °C (35.6–46.4 °F) , protected from light.
- Use within 2–4 weeks of reconstitution for optimal potency [9] .
03 · What you'll need
Supplies Needed
Keep this as a planning checklist. TNHL does not sell or verify supplies on this page, and no supplier quality claims are made here.
- Match the label on the vial before calculating.
- Use only the diluent appropriate for the compound and instructions.
- Calculator output can be translated to syringe units.
- Log dose dates, inventory, sites, and observations.
Protocol Overview
- Goal: Support cellular health, telomere maintenance, and circadian rhythm regulation [2] [3] . Standard
- Schedule: 10–20 day cycles of daily subcutaneous injections, repeated 1–2 times per year with 6–12 month breaks between cycles [4] [5] . Alternative Schedule: Extended titration protocols (8–16 weeks) for gradual dose escalation research.
- Dose Range: 5–10 mg daily during active cycles or titration phases.
- Reconstitution: 2.0 mL per 10 mg vial (5 mg/mL) for practical dosing volumes.
- Storage: Lyophilized frozen at −20 °C (−4 °F); reconstituted refrigerated at 2–8 °C (35.6–46.4 °F); avoid repeated freeze–thaw cycles [9] .
Dosing Protocol
- 5–10 mg once daily (subcutaneous injection).
- Inject once daily subcutaneously during the active cycle. After completing 10–20 days, take a break of 6–12 months before repeating.
- If values change, rerun the calculator and update the protocol so future logs match the active plan.
Storage Instructions
- Lyophilized: freeze at −20 °C (−4 °F) ; reconstituted: refrigerate at 2–8 °C (35.6–46.4 °F) ; avoid freeze–thaw cycles [9] [10] .
- Storage: Lyophilized frozen at −20 °C (−4 °F); reconstituted refrigerated at 2–8 °C (35.6–46.4 °F); avoid repeated freeze–thaw cycles [9] .
- Keep mixed and unmixed items clearly labeled so inventory and dose logs stay accurate.
04 · Good to know
Important Notes
- This Epithalon page is for educational research organization only.
- Confirm route, dose, contraindications, monitoring, storage, and cycle decisions with qualified professional guidance.
- Use sterile technique, rotate sites when applicable, and log missed doses, reactions, and inventory changes.
05 · How it works
How This Works
Epitalon (also known as Epithalon) is a synthetic tetrapeptide (Ala-Glu-Asp-Gly) developed from the naturally occurring pineal peptide epithalamin [1] .
Research indicates it may support telomerase activation, circadian rhythm regulation, and anti-aging pathways [2] [3] .
Clinical studies employ short intermittent cycles (10–20 days) repeated 1–2 times annually [4] , though extended titration protocols are also explored for research purposes.
The library page pairs that educational context with calculator-ready vial math, so users can understand the protocol structure and then track their own entered values in one place.
06 · Daily habits
Lifestyle Factors
- Keep meals, training, sleep, and recovery notes in the journal so trends are easier to review.
- Track body metrics only if they are relevant to the user's goal and approved data sources.
- Use consistent timing and logging habits so adherence data is meaningful.
07 · What to expect
Potential Benefits & Side Effects
- Observations from preclinical and clinical research literature.
08 · Technique
Injection Technique
- Wash hands with soap and water.
- Wipe the vial stopper and chosen site with alcohol, then let both dry.
- Confirm the vial concentration, calculator result, and intended draw amount before loading the syringe.
- Use the route and needle technique directed by a qualified professional or product instructions.
- For subcutaneous injections, rotate sites and inject slowly to limit leakage and irritation.
- Do not reuse needles or syringes.
- Dispose of sharps in a puncture-resistant container.
- Return stored items promptly to the correct temperature.
- Log dose, site, date, time, inventory change, and any observation.
09 · Evidence