FOXO4-DRI (10mg Vial)

01 · At a glance

Quickstart Highlights

FOXO4-DRI (also known as Proxofim) is a synthetic D-retro-inverso peptide designed to selectively induce apoptosis in senescent cells by disrupting the FOXO4–p53 protein interaction [1] [2] . When this interaction is blocked, p53 translocates to the mitochondria in senescent cells, triggering their programmed death while sparing healthy cells [1] . This educational protocol presents a once-daily subcutaneous approach using a practical dilution for precise insulin-syringe measurements.

02 · Dosing & reconstitution

Dosing & Reconstitution Guide

A practical calculator-ready setup based on the imported protocol reference. Use these values as a math starting point, then verify every entry against your own vial, diluent, and professional guidance.

Standard / Gradual Approach (3 mL = ~3.33 mg/mL)

250–500 mcg once daily (gradual titration over 16 weeks).

Reconstitution Steps

1. Draw 3.0 mL bacteriostatic water with a sterile syringe.
2. Inject slowly down the vial wall; avoid foaming.
3. Gently swirl/roll until dissolved (do not shake) [8] .
4. Label and refrigerate at 2–8 °C (35.6–46.4 °F) , protected from light.
5. For ≤10-unit (≤0.10 mL) administrations (Week 1), consider 30- or 50-unit insulin syringes for improved readability.

03 · What you'll need

Supplies Needed

Keep this as a planning checklist. TNHL does not sell or verify supplies on this page, and no supplier quality claims are made here.

Protocol Overview

• Goal: Selectively clear senescent cells via disruption of FOXO4–p53 binding [1] [2] .
• Schedule: Daily subcutaneous injections for 8–16 weeks with gradual dose escalation.
• Dose Range: 250–500 mcg daily; starting low and titrating upward over time.
• Reconstitution: 3.0 mL per 10 mg vial (~3.33 mg/mL) for accurate unit measurements.
• Storage: Lyophilized frozen at −20 °C (−4 °F); reconstituted refrigerated at 2–8 °C (35.6–46.4 °F); avoid repeated freeze–thaw [8] .

Dosing Protocol

• 250–500 mcg once daily (gradual titration over 16 weeks).
• Inject once daily subcutaneously [7] . This schedule uses the standard 3.0 mL dilution to maintain injection volumes under 0.2 mL for all doses. For ≤10-unit (≤0.10 mL) administrations, consider 30- or 50-unit insulin syringes for improved readability.
• If values change, rerun the calculator and update the protocol so future logs match the active plan.

Storage Instructions

• Lyophilized: freeze at −20 °C (−4 °F) ; after reconstitution, refrigerate at 2–8 °C (35.6–46.4 °F) ; avoid freeze–thaw cycles [8] .
• Storage: Lyophilized frozen at −20 °C (−4 °F); reconstituted refrigerated at 2–8 °C (35.6–46.4 °F); avoid repeated freeze–thaw [8] .
• Keep mixed and unmixed items clearly labeled so inventory and dose logs stay accurate.

04 · Good to know

Important Notes

• Practical considerations for consistency and safety.
• Use new sterile insulin syringes (typically 29–31 gauge, 0.5 inch needle) for each injection [9] ; dispose in a sharps container.
• Rotate injection sites (abdomen, thighs, upper arms) at least 1 inch apart to reduce local irritation [10] .
• Clean the injection site with an alcohol swab and allow to dry before injecting [9] .
• Inject slowly and steadily; no need to aspirate for subcutaneous injections [11] .
• Document daily dose and site rotation to maintain consistency throughout the protocol.

05 · How it works

How This Works

FOXO4-DRI (also known as Proxofim) is a synthetic D-retro-inverso peptide designed to selectively induce apoptosis in senescent cells by disrupting the FOXO4–p53 protein interaction [1] [2] .

When this interaction is blocked, p53 translocates to the mitochondria in senescent cells, triggering their programmed death while sparing healthy cells [1] .

This educational protocol presents a once-daily subcutaneous approach using a practical dilution for precise insulin-syringe measurements.

The library page pairs that educational context with calculator-ready vial math, so users can understand the protocol structure and then track their own entered values in one place.

06 · Daily habits

Lifestyle Factors

• Keep meals, training, sleep, and recovery notes in the journal so trends are easier to review.
• Track body metrics only if they are relevant to the user's goal and approved data sources.
• Use consistent timing and logging habits so adherence data is meaningful.

07 · What to expect

Potential Benefits & Side Effects

• FOX04-DRI observations should be recorded conservatively: expected research endpoints, tolerability notes, injection-site reactions when applicable, sleep, appetite, energy, mood, and any adverse or unusual response.
• This section is not a claim of therapeutic benefit.

08 · Technique

Injection Technique

09 · Evidence

References