Protocol
HCG (5000iu Vial)
Complete dosage protocol and reconstitution guide for HCG (5000iu Vial).
Calculator preset
Run the vial math before tracking.
At 2,500 IU/mL, 1 unit = 25 IU on a U-100 syringe; 500 IU = 20 units (0.20 mL) . About 10 doses per vial .
01 · At a glance
Quickstart Highlights
Human Chorionic Gonadotropin (HCG) is a glycoprotein hormone that mimics luteinizing hormone (LH) by binding to LH receptors in the gonads [1] . With a 36‑hour half‑life (compared to LH’s 30 minutes), HCG provides sustained stimulation of testosterone production in men and ovulation induction in women [2] . This educational protocol presents a practical three‑times‑weekly subcutaneous approach for maintaining testicular function and fertility.
02 · Dosing & reconstitution
Dosing & Reconstitution Guide
A practical calculator-ready setup based on the imported protocol reference. Use these values as a math starting point, then verify every entry against your own vial, diluent, and professional guidance.
Standard Protocol (2 mL = 2,500 IU/mL)
Reference dose shown on this page: See guide.
Reconstitution Steps
- Draw 2.0 mL bacteriostatic water with a sterile syringe.
- Inject slowly down the vial wall; avoid foaming or vigorous shaking.
- Gently swirl or roll until the powder fully dissolves (clear solution).
- Label vial with reconstitution date and concentration (2,500 IU/mL); refrigerate immediately at 2–8 °C (35.6–46.4 °F) .
- Dosing Calculations: 250 IU = 10 units (0.10 mL) 500 IU = 20 units (0.20 mL) 1,000 IU = 40 units (0.40 mL)
03 · What you'll need
Supplies Needed
Keep this as a planning checklist. TNHL does not sell or verify supplies on this page, and no supplier quality claims are made here.
- 12 weeks (1,500 IU/week × 12 = 18,000 IU total): 4 vials
- 16 weeks (1,500 IU/week × 16 = 24,000 IU total): 5 vials
- 8 weeks: 24 syringes
- 12 weeks: 36 syringes
- 16 weeks: 48 syringes
- 8 weeks (3 vials): 6 mL → 1 × 10 mL bottle
- 12 weeks (4 vials): 8 mL → 1 × 10 mL bottle
- 16 weeks (5 vials): 10 mL → 1 × 10 mL bottle
- Per week: 6 swabs (2
- per injection × 3 injections)
- 8 weeks: 48 swabs → recommend 1 × 100‑count box
- 12 weeks: 72 swabs → recommend 1 × 100‑count box
Protocol Overview
- Goal: Maintain testicular function and fertility during testosterone replacement therapy or restore endogenous testosterone production post‑cycle [3] [7] .
- Schedule: Subcutaneous injections 3 times weekly for 8–16 weeks.
- Dose Range: Standard maintenance: 500 IU per injection (1,500 IU/week); high‑dose recovery: 1,500–2,500 IU per injection .
- Reconstitution: 2.0 mL per 5000 IU vial (2,500 IU/mL) for precise insulin‑syringe measurements.
- Storage: Refrigerate lyophilized and reconstituted vials; reconstituted solution stable up to 60 days.
Dosing Protocol
- Reference dose shown on this page: See guide.
- Inject 3 times weekly subcutaneously (e.g., Monday/Wednesday/Friday). This yields a total weekly dose of 1,500 IU . This schedule maintains intratesticular testosterone during exogenous testosterone therapy and supports fertility preservation [3] [4] .
- If values change, rerun the calculator and update the protocol so future logs match the active plan.
Storage Instructions
- Lyophilized: refrigerate at 2–8 °C (35.6–46.4 °F) ; after reconstitution, refrigerate at 2–8 °C (35.6–46.4 °F) for up to 60 days .
- Storage: Refrigerate lyophilized and reconstituted vials; reconstituted solution stable up to 60 days.
- Keep mixed and unmixed items clearly labeled so inventory and dose logs stay accurate.
04 · Good to know
Important Notes
- This HCG page is for educational research organization only.
- Confirm route, dose, contraindications, monitoring, storage, and cycle decisions with qualified professional guidance.
- Use sterile technique, rotate sites when applicable, and log missed doses, reactions, and inventory changes.
05 · How it works
How This Works
Human Chorionic Gonadotropin (HCG) is a glycoprotein hormone that mimics luteinizing hormone (LH) by binding to LH receptors in the gonads [1] .
With a 36‑hour half‑life (compared to LH’s 30 minutes), HCG provides sustained stimulation of testosterone production in men and ovulation induction in women [2] .
This educational protocol presents a practical three‑times‑weekly subcutaneous approach for maintaining testicular function and fertility.
The library page pairs that educational context with calculator-ready vial math, so users can understand the protocol structure and then track their own entered values in one place.
06 · Daily habits
Lifestyle Factors
- Keep meals, training, sleep, and recovery notes in the journal so trends are easier to review.
- Track body metrics only if they are relevant to the user's goal and approved data sources.
- Use consistent timing and logging habits so adherence data is meaningful.
07 · What to expect
Potential Benefits & Side Effects
- Documented effects from clinical literature and medical practice.
08 · Technique
Injection Technique
- Wash hands with soap and water.
- Wipe the vial stopper and chosen site with alcohol, then let both dry.
- Confirm the vial concentration, calculator result, and intended draw amount before loading the syringe.
- Use the route and needle technique directed by a qualified professional or product instructions.
- For subcutaneous injections, rotate sites and inject slowly to limit leakage and irritation.
- Do not reuse needles or syringes.
- Dispose of sharps in a puncture-resistant container.
- Return stored items promptly to the correct temperature.
- Log dose, site, date, time, inventory change, and any observation.
09 · Evidence