Protocol
PE-22-28 (10mg Vial)
Complete dosage protocol and reconstitution guide for PE-22-28 (10mg Vial).
Calculator preset
Run the vial math before tracking.
At ~3.33 mg/mL, 1 unit ~ 33.3 mcg ; 50 mcg ~ 1.5 units and 200 mcg ~ 6 units on a U-100 syringe.
01 · At a glance
Quickstart Highlights
PE-22-28 is a synthetic heptapeptide (sequence: GVSWGLR) derived from the sortilin propeptide, engineered as a potent and selective antagonist of TREK-1 potassium channels [4] . Preclinical studies demonstrate rapid antidepressant-like effects, enhanced neurogenesis, and neuroprotection with a favorable safety profile showing no cardiac or metabolic side effects [2] [3] . This educational protocol presents a once-daily subcutaneous approach with gradual titration.
02 · Dosing & reconstitution
Dosing & Reconstitution Guide
A practical calculator-ready setup based on the imported protocol reference. Use these values as a math starting point, then verify every entry against your own vial, diluent, and professional guidance.
Standard / Gradual Approach (3 mL = ~3.33 mg/mL)
50–200 µg once daily (gradual titration over 8–16 weeks).
Reconstitution Steps
- Draw 3.0 mL bacteriostatic water with a sterile syringe.
- Inject slowly down the vial wall; avoid foaming.
- Gently swirl/roll until dissolved (do not shake).
- Label and refrigerate at 2–8 °C (35.6–46.4 °F) , protected from light.
03 · What you'll need
Supplies Needed
Keep this as a planning checklist. TNHL does not sell or verify supplies on this page, and no supplier quality claims are made here.
- 12 weeks ≈ 3 vials
- 16 weeks ≈ 4 vials
- 8 weeks: 56 syringes
- 12 weeks: 84 syringes
- 16 weeks: 112 syringes
- 8 weeks (2 vials): 6 mL → 1 × 10 mL bottle
- 12 weeks (3 vials): 9 mL → 1 × 10 mL bottle
- 16 weeks (4 vials): 12 mL → 2 × 10 mL bottles
- Per week: 14 swabs (2/day)
- 8 weeks: 112 swabs → recommend 2 × 100-count boxes
- 12 weeks: 168 swabs → recommend 2 × 100-count boxes
- 16 weeks: 224 swabs → recommend 3 × 100-count boxes
Protocol Overview
- Goal: Support rapid neuroplasticity, mood regulation, and neuroprotection through selective TREK-1 inhibition [2] [4] .
- Schedule: Daily subcutaneous injections for 12–16 weeks (8 weeks minimum).
- Dose Range: 50–200 µg daily with conservative titration; many find 100–150 µg adequate.
- Reconstitution: 3.0 mL per 10 mg vial (~3.33 mg/mL) for precise low-volume measurements.
- Storage: Lyophilized frozen; reconstituted refrigerated; replace vials every 4 weeks.
Dosing Protocol
- Suggested conservative daily titration approach.
- Start: 50 µg daily for Weeks 1–2 to assess tolerance.
- Increase: 100 µg daily for Weeks 3–8 (standard maintenance dose).
- Optional Titration: 150 µg (Weeks 9–12) or 200 µg (Weeks 13–16) only if well-tolerated and additional effect desired.
- Frequency: Once per day (subcutaneous).
- Cycle Length: 12–16 weeks; 8 weeks minimum.
- Timing: Consistent daily time; rotate injection sites systematically.
Storage Instructions
- Proper storage preserves peptide quality and potency.
- Lyophilized: Store at −20 °C (−4 °F) in dry, dark conditions; minimize moisture exposure.
- Reconstituted: Refrigerate at 2–8 °C (35.6–46.4 °F); use within 4 weeks for optimal potency.
- Allow vials to reach room temperature before opening to reduce condensation; protect from light.
- Replace with fresh vial every 4 weeks even if peptide remains; bacteriostatic water sterility is guaranteed for 28 days after first puncture [10] .
04 · Good to know
Important Notes
- This PE-22-28 page is for educational research organization only.
- Confirm route, dose, contraindications, monitoring, storage, and cycle decisions with qualified professional guidance.
- Use sterile technique, rotate sites when applicable, and log missed doses, reactions, and inventory changes.
05 · How it works
How This Works
PE-22-28 is a synthetic heptapeptide (sequence: GVSWGLR) derived from the sortilin propeptide, engineered as a potent and selective antagonist of TREK-1 potassium channels [4] .
Preclinical studies demonstrate rapid antidepressant-like effects, enhanced neurogenesis, and neuroprotection with a favorable safety profile showing no cardiac or metabolic side effects [2] [3] .
This educational protocol presents a once-daily subcutaneous approach with gradual titration.
The library page pairs that educational context with calculator-ready vial math, so users can understand the protocol structure and then track their own entered values in one place.
06 · Daily habits
Lifestyle Factors
- Keep meals, training, sleep, and recovery notes in the journal so trends are easier to review.
- Track body metrics only if they are relevant to the user's goal and approved data sources.
- Use consistent timing and logging habits so adherence data is meaningful.
07 · What to expect
Potential Benefits & Side Effects
- PE-22-28 observations should be recorded conservatively: expected research endpoints, tolerability notes, injection-site reactions when applicable, sleep, appetite, energy, mood, and any adverse or unusual response.
- This section is not a claim of therapeutic benefit.
08 · Technique
Injection Technique
- Wash hands with soap and water.
- Wipe the vial stopper and chosen site with alcohol, then let both dry.
- Confirm the vial concentration, calculator result, and intended draw amount before loading the syringe.
- Use the route and needle technique directed by a qualified professional or product instructions.
- For subcutaneous injections, rotate sites and inject slowly to limit leakage and irritation.
- Do not reuse needles or syringes.
- Dispose of sharps in a puncture-resistant container.
- Return stored items promptly to the correct temperature.
- Log dose, site, date, time, inventory change, and any observation.
09 · Evidence