Protocol
Pinealon (20mg Vial)
Complete dosage protocol and reconstitution guide for Pinealon (20mg Vial).
Calculator preset
Run the vial math before tracking.
At ~6.67 mg/mL, 1 unit ~ 66.7 mcg ; 200 mcg ~ 3 units and 500 mcg ~ 7.5 units on a U-100 syringe.
01 · At a glance
Quickstart Highlights
Pinealon (also known as EDR ; sequence Glu‑Asp‑Arg ) is an ultrashort tripeptide investigated for neuroprotective and epigenetic regulatory effects, with most peer‑reviewed data from in vitro and animal studies and limited human observations [1] [2] [3] [4] [5] . Published literature frequently describes oral administration in clinical contexts; subcutaneous (SC) human dosing is not established and the SC framework below is educational for research settings only [4] [5] .
02 · Dosing & reconstitution
Dosing & Reconstitution Guide
A practical calculator-ready setup based on the imported protocol reference. Use these values as a math starting point, then verify every entry against your own vial, diluent, and professional guidance.
Standard / Gradual Approach (3 mL = ~6.67 mg/mL)
(educational): 200–500 mcg once daily with gradual titration (SC route not defined in clinical trials; see Evidence note above). Insulin‑syringe math: At 6.67 mg/mL, 1 unit = 0.01 mL ≈ 66.7 mcg on a U‑100 insulin syringe.
Reconstitution Steps
- Draw 3.0 mL bacteriostatic water with a sterile syringe.
- Inject slowly down the vial wall; avoid foaming.
- Gently swirl/roll until dissolved (do not shake).
- Label and refrigerate at 2–8 °C (35.6–46.4 °F) , protected from light [12] [13] .
03 · What you'll need
Supplies Needed
Keep this as a planning checklist. TNHL does not sell or verify supplies on this page, and no supplier quality claims are made here.
- 12 weeks ≈ 2 vials (≈33.6 mg total)
- 16 weeks ≈ 3 vials (≈47.6 mg total)
- 8 weeks: 56 syringes
- 12 weeks: 84 syringes
- 16 weeks: 112 syringes
- 8 weeks (1 vial): 3 mL → 1 × 10 mL bottle
- 12 weeks (2 vials): 6 mL → 1 × 10 mL bottle
- 16 weeks (3 vials): 9 mL → 1 × 10 mL bottle
- Per week: 14 swabs (2/day)
- 8 weeks: 112 swabs → recommend 2 × 100‑count boxes
- 12 weeks: 168 swabs → recommend 2 × 100‑count boxes
- 16 weeks: 224 swabs → recommend 3 × 100‑count boxes
Protocol Overview
- Goal: Study neuroprotective and stress‑response pathways influenced by EDR/Pinealon [1] [3] [5] .
- Schedule: Once‑daily subcutaneous injections for 8–12 weeks (optional extension to 16 weeks); note oral dosing dominates human literature [4] [5] .
- Dose Range: 200–500 mcg daily with gradual titration (educational; not derived from SC clinical trials).
- Reconstitution: 3.0 mL per 20 mg vial (~6.67 mg/mL) for clear syringe conversions.
- Storage: Lyophilized at −20 °C (−4 °F); reconstituted at 2–8 °C (35.6–46.4 °F); avoid repeated freeze–thaw [12] [13] .
Dosing Protocol
- Suggested titration for measurement practice and exposure control (research‑only).
- Start: 200–300 mcg daily; increase by ~100 mcg every 1–2 weeks as tolerated.
- Target: 400–500 mcg daily by Weeks 5–12.
- Frequency: Once per day (subcutaneous).
- Cycle Length: 8–12 weeks; optional extension to 16 weeks.
- Timing: Any consistent time; rotate injection sites.
Storage Instructions
- Lyophilized: store at −20 °C (−4 °F) protected from light; after reconstitution, refrigerate at 2–8 °C (35.6–46.4 °F) ; minimize freeze–thaw cycles [12] [13] .
- Storage: Lyophilized at −20 °C (−4 °F); reconstituted at 2–8 °C (35.6–46.4 °F); avoid repeated freeze–thaw [12] [13] .
- Keep mixed and unmixed items clearly labeled so inventory and dose logs stay accurate.
04 · Good to know
Important Notes
- This Pinealon page is for educational research organization only.
- Confirm route, dose, contraindications, monitoring, storage, and cycle decisions with qualified professional guidance.
- Use sterile technique, rotate sites when applicable, and log missed doses, reactions, and inventory changes.
05 · How it works
How This Works
Pinealon (also known as EDR ; sequence Glu‑Asp‑Arg ) is an ultrashort tripeptide investigated for neuroprotective and epigenetic regulatory effects, with most peer‑reviewed data from in vitro and animal studies and limited human observations [1] [2] [3] [4] [5] .
Published literature frequently describes oral administration in clinical contexts; subcutaneous (SC) human dosing is not established and the SC framework below is educational for research settings only [4] [5] .
Reconstitute: Add 3.0 mL bacteriostatic water → ~ 6.67 mg/mL concentration.
The library page pairs that educational context with calculator-ready vial math, so users can understand the protocol structure and then track their own entered values in one place.
06 · Daily habits
Lifestyle Factors
- Keep meals, training, sleep, and recovery notes in the journal so trends are easier to review.
- Track body metrics only if they are relevant to the user's goal and approved data sources.
- Use consistent timing and logging habits so adherence data is meaningful.
07 · What to expect
Potential Benefits & Side Effects
- Observations from preclinical and review literature.
- Neuroprotective and anti‑oxidative signals in vitro and in vivo models [1] [2] .
- Epigenetic modulation and preservation of neuronal structures (spines/synapses) in models [5] [6] [7] .
- Human evidence remains limited and heterogeneous; safety/tolerability for SC use is not characterized in controlled trials [4] [5] .
- Subcutaneous injections may cause mild local reactions (redness/itch); rotate sites to avoid lipohypertrophy [8] [11] .
08 · Technique
Injection Technique
- Wash hands with soap and water.
- Wipe the vial stopper and chosen site with alcohol, then let both dry.
- Confirm the vial concentration, calculator result, and intended draw amount before loading the syringe.
- Use the route and needle technique directed by a qualified professional or product instructions.
- For subcutaneous injections, rotate sites and inject slowly to limit leakage and irritation.
- Do not reuse needles or syringes.
- Dispose of sharps in a puncture-resistant container.
- Return stored items promptly to the correct temperature.
- Log dose, site, date, time, inventory change, and any observation.
09 · Evidence