Protocol
Semax (10mg Vial)
Complete dosage protocol and reconstitution guide for Semax (10mg Vial).
Calculator preset
Run the vial math before tracking.
At ~3.33 mg/mL, 1 unit ~ 33.3 mcg ; 500 mcg ~ 15 units and 1000 mcg ~ 30 units on a U-100 syringe.
01 · At a glance
Quickstart Highlights
Semax is a synthetic heptapeptide analog of ACTH(4–10) developed in Russia and studied primarily for cognitive enhancement and neuroprotection [1] [2] . While intranasal administration is most common in clinical literature, subcutaneous injection offers a convenient once‑daily alternative for research purposes [1] [4] . This educational protocol presents a practical SC approach using straightforward reconstitution for accurate insulin‑syringe measurements.
02 · Dosing & reconstitution
Dosing & Reconstitution Guide
A practical calculator-ready setup based on the imported protocol reference. Use these values as a math starting point, then verify every entry against your own vial, diluent, and professional guidance.
Standard / Gradual Approach (3 mL = ~3.33 mg/mL)
300–800 mcg once daily (gradual titration recommended).
Reconstitution Steps
- Draw 3.0 mL bacteriostatic water with a sterile syringe.
- Inject slowly down the vial wall; avoid foaming.
- Gently swirl/roll until dissolved (do not shake vigorously).
- Label and refrigerate at 2–8 °C (35.6–46.4 °F) , protected from light [2] .
03 · What you'll need
Supplies Needed
Keep this as a planning checklist. TNHL does not sell or verify supplies on this page, and no supplier quality claims are made here.
- 12 weeks ≈ 6 vials
- 16 weeks ≈ 8 vials
- 8 weeks: 56 syringes
- 12 weeks: 84 syringes
- 16 weeks: 112 syringes
- 8 weeks (4 vials): 12 mL → 2 × 10 mL bottles
- 12 weeks (6 vials): 18 mL → 2 × 10 mL bottles
- 16 weeks (8 vials): 24 mL → 3 × 10 mL bottles
- Per week: 14 swabs (2/day)
- 8 weeks: 112 swabs → recommend 2 × 100‑count boxes
- 12 weeks: 168 swabs → recommend 2 × 100‑count boxes
- 16 weeks: 224 swabs → recommend 3 × 100‑count boxes
Protocol Overview
- Goal: Support cognitive function, attention, and neuroprotective pathways studied in clinical literature [2] [5] .
- Schedule: Daily subcutaneous injections for 8 weeks (extend to 12–16 weeks with cycling if desired) [4] .
- Dose Range: 300–800 mcg daily with gradual titration; aligns with typical human nootropic dosing ranges [2] [3] .
- Reconstitution: 3.0 mL per 10 mg vial (~3.33 mg/mL) for accurate unit measurements.
- Storage: Lyophilized frozen; reconstituted refrigerated; use within 30 days [2] [9] .
Dosing Protocol
- Suggested daily titration approach.
- Start: 300 mcg daily; increase by ~100–200 mcg every 1–2 weeks as tolerated.
- Target: 600–800 mcg daily by Weeks 5–8; adjust based on individual response.
- Frequency: Once per day (subcutaneous) [1] [4] .
- Cycle Length: 8 weeks continuous; optional extension to 12–16 weeks with off‑periods (e.g., 6 weeks on, 2 weeks off) [4] .
- Timing: Any consistent time; rotate injection sites systematically.
Storage Instructions
- Lyophilized: freeze at −20 °C (−4 °F) ; after reconstitution, refrigerate at 2–8 °C (35.6–46.4 °F) ; avoid freeze–thaw cycles [2] [9] .
- Storage: Lyophilized frozen; reconstituted refrigerated; use within 30 days [2] [9] .
- Keep mixed and unmixed items clearly labeled so inventory and dose logs stay accurate.
04 · Good to know
Important Notes
- Practical considerations for consistency and safety.
- Use new sterile insulin syringes for each injection; dispose in a sharps container [7] .
- Rotate injection sites (abdomen, thighs, upper arms) at least 1–2 inches from previous sites to reduce local irritation [7] .
- Inject slowly; wait a few seconds before withdrawing the needle.
- Document daily dose, injection time, and site rotation to maintain consistency.
- Most human data are for 4–8 weeks of continuous use; longer protocols should incorporate rest periods [4] .
05 · How it works
How This Works
Semax is a synthetic heptapeptide analog of ACTH(4–10) developed in Russia and studied primarily for cognitive enhancement and neuroprotection [1] [2] .
While intranasal administration is most common in clinical literature, subcutaneous injection offers a convenient once‑daily alternative for research purposes [1] [4] .
This educational protocol presents a practical SC approach using straightforward reconstitution for accurate insulin‑syringe measurements.
The library page pairs that educational context with calculator-ready vial math, so users can understand the protocol structure and then track their own entered values in one place.
06 · Daily habits
Lifestyle Factors
- Keep meals, training, sleep, and recovery notes in the journal so trends are easier to review.
- Track body metrics only if they are relevant to the user's goal and approved data sources.
- Use consistent timing and logging habits so adherence data is meaningful.
07 · What to expect
Potential Benefits & Side Effects
- Semax observations should be recorded conservatively: expected research endpoints, tolerability notes, injection-site reactions when applicable, sleep, appetite, energy, mood, and any adverse or unusual response.
- This section is not a claim of therapeutic benefit.
08 · Technique
Injection Technique
- Wash hands with soap and water.
- Wipe the vial stopper and chosen site with alcohol, then let both dry.
- Confirm the vial concentration, calculator result, and intended draw amount before loading the syringe.
- Use the route and needle technique directed by a qualified professional or product instructions.
- For subcutaneous injections, rotate sites and inject slowly to limit leakage and irritation.
- Do not reuse needles or syringes.
- Dispose of sharps in a puncture-resistant container.
- Return stored items promptly to the correct temperature.
- Log dose, site, date, time, inventory change, and any observation.
09 · Evidence
References
Related pages