🛡️ Dipeptide Bioregulator • Thymic/Immune

Vilon (KE)

Synthetic dipeptide (Lys-Glu) thymic bioregulator studied for immune modulation, IL-2 gene upregulation, chromatin remodeling in aged cells, and lifespan extension in animal models.

Sequence K-E MW ~275.3 Da CAS 45234-02-4

⚡ Executive Summary

Vilon (KE: Lys-Glu) is a synthetic dipeptide from the thymic peptide bioregulator family studied for immune modulation and cellular “rejuvenation” effects. In vitro and animal studies report IL-2 gene upregulation, chromatin decondensation in aged lymphocytes, lifespan extension in mice, and GI enzyme restoration in aged rats. Research use only — not FDA approved.

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01 Overview 02 Properties 03 Mechanism 04 Outcomes 05 Handling 06 Comparison 📋

Overview

🛡️ What is Vilon?

Vilon is an ultra-short dipeptide (Lys-Glu) that appears to “retune” immune and gene-expression programs rather than forcing large, drug-like effects.

It belongs to the thymic peptide bioregulator family studied for immunomodulatory and geroprotective properties.

🎯 Key Actions

• 🧬 IL-2 upregulation — T-cell activation
• 🔓 Chromatin decondensation — aged cells
• ⏳ Lifespan extension — mouse models

💡

Key takeaway: Vilon = immune/epigenetic tuning (IL-2 + chromatin). This distinguishes it from Epitalon (telomerase/circadian) and Thymogen/Thymalin (broader thymic immune support).

⚠️

Research only: Vilon is NOT an FDA-approved therapy. Human-scale trials are limited. Handle as a research compound only.

🔬

Entity Properties

Aliases	Vilon, KE, L-Lys-L-Glu, lysylglutamic acid
Sequence	Lys-Glu (K-E)
Length	2 amino acids (dipeptide)
Molecular Formula	C₁₁H₂₁N₃O₅
Molecular Weight	~275.3 Da
CAS Number	45234-02-4 (dipeptide)
PubChem CID	7010502
Family	Thymic peptide bioregulator (immunomodulatory)
Diluent(s)	Sterile saline or bacteriostatic water
Concentration	20 mg + 2 mL = 10 mg/mL (example)
Storage (dry)	≤ −20°C, protected from light; stable long-term
Storage (solution)	2–8°C short-term; avoid freeze-thaw

⚠️

CAS note: Distinguish dipeptide Vilon (CAS 45234-02-4) from the amino-acid salt L-lysine·L-glutamate (CAS 5408-52-6). These are different compounds.

⚙️

Mechanism of Action

🧠 How does Vilon work?

Vilon acts via two converging mechanisms: (1) IL-2 gene upregulation in lymphocytes — enhancing T-cell activation and expansion, and (2) chromatin decondensation in aged cells — re-opening access to silenced genes.

This “retuning” of immune and gene-expression programs is concentration- and time-dependent, favoring homeostasis rather than forcing large drug-like effects.

🔄 Two-Phase Action Model

Vilon’s actions fit a two-phase model that helps explain why short cycles produce durable, system-level changes even after dosing stops:

1

Fast Transcriptional Nudges

IL-2 mRNA rise, T-cell activation signals

2

Slower Chromatin Remodeling

Re-opens access to growth/repair gene programs

🧬 IL-2 Upregulation

Increases IL-2 mRNA in splenic lymphocytes → enhanced T-cell proliferation and function

🔓 Chromatin Opening

Deheterochromatinization in aged cells → more transcriptionally active euchromatin

📈 rRNA Activation

Reactivates nucleolar organizer regions → ribosomal RNA synthesis and protein production

📊

Research Outcomes

🔬 Key Preclinical Findings

🛡️

Immunomodulation

IL-2 gene upregulation in lymphocytes — direct mechanism for T-cell activation. Modulates macrophage proliferative activity.

🧬

Epigenetic Rejuvenation

Chromatin decondensation in elderly donors’ lymphocytes. Reactivates ribosomal genes, increases NOR activity.

⏳

Longevity Signals (Mice)

Female CBA mice: extended lifespan, increased endurance, reduced spontaneous tumors, no adverse effects.

🫁

GI/Metabolic Support

Aged rats: increased brush-border enzymes (maltase, invertase, ALP), improved glucose/glycine transport.

💡

Age-dependent effects: Vilon’s effects are especially pronounced in older models/subjects, effectively narrowing age-related gaps in immune function, chromatin state, and digestive capacity.

⚠️

Evidence grade: All data is preclinical (cell cultures, animal models). Human longevity/clinical data are not available. Findings are promising but require human trials for validation.

🧪

Research Handling

📘

Research use only. Handling details are provided for laboratory contexts. Vilon is not an FDA-approved therapy — do not construe as directions for clinical use.

1

Plan Concentration

20 mg vial + 2 mL diluent = 10 mg/mL. Makes downstream aliquoting simple.

2

Reconstitute Sterile

Use sterile saline or bac water. Direct down vial wall; swirl don’t shake. Let fully dissolve.

3

Aliquot

Split into micro-aliquots sized to single day/experiment. Minimizes freeze-thaw cycles.

4

Store Properly

Lyophilized: ≤ −20°C long-term. Solutions: 2–8°C short-term. Use promptly.

5

Document Everything

Label each aliquot: peptide, lot, concentration, date. Record storage conditions.

6

Pre-register Endpoints

Define primary readouts: IL-2 mRNA, chromatin marks, AgNOR counts, enzyme activity.

⚖️

Comparison

Choose Vilon for immune tuning + age-linked gene-expression support; Epitalon for telomerase/circadian endpoints; Thymogen/Thymalin for broader thymic immune normalization.

Vilon

KE (Lys-Glu) 🛡️ Immune + 🧬 Epigenetic

IL-2 gene upregulation. Chromatin decondensation in aged lymphocytes. Lifespan extension in mice. GI enzyme restoration in aged rats.

Epitalon

AEDG (Ala-Glu-Asp-Gly) 🧬 Telomerase + ⏰ Circadian

Telomerase activation. Telomere elongation in human somatic cells. Circadian/neuroendocrine support. Pineal bioregulator.

Thymogen

EW (Glu-Trp) 🛡️ Immune Differentiation

Immunocorrector. Clinical use in some countries. Vaccine response support. Geroprotective signals in rodents.

Thymalin

Thymic peptide complex 🛡️ Broad Immune Support

Polypeptide drug. Broad immune normalization. Used clinically in select countries. COVID-19 immunocorrection studies.

❓

FAQ

What is Vilon? A synthetic dipeptide (Lys-Glu) studied as a thymic peptide bioregulator that can increase IL-2 gene expression in lymphocytes and remodel age-related chromatin in immune cells. Research use only. Is Vilon the same as “KE”? Yes — “KE” is the short-name code for the Lys-Glu dipeptide known as Vilon. In the bioregulator literature, KE (Lys-Glu) is repeatedly referred to as Vilon. How does Vilon work? Via two converging mechanisms: (1) IL-2 gene upregulation in lymphocytes enhancing T-cell activation, and (2) chromatin decondensation in aged cells re-opening access to silenced genes for repair/growth programs. Does Vilon increase lifespan? In female CBA mice, Vilon extended lifespan and reduced spontaneous tumor formation when given chronically from mid-life, without noted adverse effects. Human longevity data are not available. How is Vilon different from Epitalon? Epitalon (AEDG) primarily shows telomerase/telomere and circadian effects, while Vilon (Lys-Glu) shows strong immune and epigenetic-chromatin effects. They’re often considered complementary in aging research. Is Vilon safe? Preclinical data suggest a favorable safety profile, including a long-term mouse study with no adverse developmental effects alongside pro-longevity signals. Human-scale trials are limited — remains research-only. ✅

Bottom line: Vilon (Lys-Glu) is a compact dipeptide with outsized potential in immune and epigenetic research, showing IL-2 upregulation, chromatin remodeling, lifespan extension, and GI enzyme restoration in aged models. Its two-phase action (fast transcriptional nudges → slower chromatin remodeling) helps explain durable effects from short cycles. Research use only.